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foldseek-multimer-analysis

This repository contains the scripts needed for reproducing the results of Rapid and Sensitive Protein Complex Alignment with Foldseek-Multimer by Kim et al. 2024.

Obtaining Datasets

Download

The following script downloads files from Zenodo, organizes them and unites those of them that had to be split due to file size limitations:

foldseek-multimer-analysis/datasets/download.sh
mkdir foldseek-multimer-analysis/datasets/CRISPR/PDBs

Download CIF files from wwpdb.org and put them in foldseek-multimer-analysis/datasets/CRISPR/PDBs

Remove
foldseek-multimer-analysis/datasets/remove.sh

Similar Pairs Benchmark

foldseek-multimer-analysis/similar_pairs_benchmark/run_FS-MM_similar_pairs_benchmark.sh [Foldseek PATH] [TEMP DIR]
foldseek-multimer-analysis/similar_pairs_benchmark/run_US-align_similar_pairs_benchmark.sh [US-align PATH] [TEMP DIR]
foldseek-multimer-analysis/similar_pairs_benchmark/tot.py [TEMP DIR] [RESULT TSV]

CRISPR Benchmark

Createdb
foldseek-multimer-analysis/CRISPR_benchmark/run_CRISPR_createdb.sh [FOLDSEEK PATH] [THREADS] [DIR for DATABASE FILES]
Foldseek
foldseek-multimer-analysis/CRISPR_benchmark/run_CRISPR_benchmark.sh [FOLDSEEK PATH] [DIR for DATABASE FILES] [TEMP DIR]
US-align: runtime with 1,000 targets
foldseek-multimer-analysis/CRISPR_benchmark/run_US-align_CRISPR_runtime.sh [US-align PATH] [TEMP DIR] [OUTPUT FILE]
US-align: all positive results
foldseek-multimer-analysis/CRISPR_benchmark/run_US-align_CRISPR_whole.sh [US-align PATH] [TEMP DIR] [OUTPUT PATH] [THREADS]

3DComplexV7 Benchmark

Createdb
foldseek-multimer-analysis/3DComplexV7_benchmark/run_3DComplexV7_createdb.sh [FOLDSEEK PATH] [THREADS] [DIR for DATABASE FILES]
Database Search
foldseek-multimer-analysis/3DComplexV7_benchmark/run_database_search.sh [US-align PATH] [FOLDSEEK PATH] [DIR for DATABASE FILES] [TSV OUTPUT PATH] [TEMP DIR]
Foldseek-MM Benchmark
foldseek-multimer-analysis/3DComplexV7_benchmark/run_FS-MM_3DComplexV7_benchmark.sh [FOLDSEEK PATH] [DIR for DATABASE FILES] [CSV OUTPUT] [THREADS] [TEMP DIR]
foldseek-multimer-analysis/3DComplexV7_benchmark/tot.py foldseek-multimer-analysis/datasets/3DComplexV7/QSalign_pairs.csv [CSV INPUT] [TSV OUTPUT PATH]

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