Loxodes magnus MIC and MAC nuclei were sorted with FACS. Nucleosomal DNA was prepared with Atlantis dsDNAse digest.
- Map nucleosomal DNA library to Loxodes magnus MAC reference assembly (Falcon assembler)
- Also filter assembly to exclude low-complexity repeat regions previously annotated with TRF (min 1 kbp region)
- Evaluate coverage and nucleosomal DNA fragment length distribution
- Global phaseogram of nucleosome positioning relative to each other
- Phaseogram of nucleosome positioning relative to starts of gene features (TSS data not available)