From 8c64601f89f9349d8b056f2e29e50ec7ce1a87a7 Mon Sep 17 00:00:00 2001 From: atrigila <18577080+atrigila@users.noreply.github.com> Date: Tue, 12 Nov 2024 16:36:07 +0000 Subject: [PATCH 1/4] update docs --- docs/usage.md | 4 ++++ 1 file changed, 4 insertions(+) diff --git a/docs/usage.md b/docs/usage.md index 46fbe6eb..3549542a 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -45,6 +45,10 @@ If MirGeneDB should be used instead it needs to be specified using `--mirgenedb` - `mirgenedb_mature`: This parameter should point to the FASTA file containing mature miRNA sequences. The file can be manually downloaded from [MirGeneDB](https://mirgenedb.org/download). - `mirgenedb_hairpin`: This parameter should point to the FASTA file containing precursor miRNA sequences. Note that MirGeneDB does not offer a dedicated hairpin file, but the precursor sequences can be downloaded from [MirGeneDB](https://mirgenedb.org/download) and used instead. +See examples in: [the test-datasets repository of nf-core](https://github.com/nf-core/test-datasets/tree/smrnaseq/MirGeneDB). + +> [!NOTE] > `mirtop` is hard-coded to use the `pre` sequences rather than `pri`. Users must provide `pre` files from the start to ensure consistency between the FASTA and GFF files, as the coordinates in the GFF file are referenced to `pre` sequences. This also ensures that names in the BAM file will match those in the GFF. + ### Genome - `fasta`: the reference genome FASTA file From b1513c60bfadc0c973db578dfe3166181a9085e4 Mon Sep 17 00:00:00 2001 From: atrigila <18577080+atrigila@users.noreply.github.com> Date: Tue, 12 Nov 2024 16:36:15 +0000 Subject: [PATCH 2/4] update changelog --- CHANGELOG.md | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index ae8fdc78..e2ee8bc3 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -5,7 +5,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ## Dev vTBD - TBD - TBD -- [[#477]](https://github.com/nf-core/smrnaseq/issues/481) - Fix [MIRTOP_STATS IndexError](https://github.com/nf-core/smrnaseq/issues/477) - Fix mirtop process execution when mirgenedb is used. +- [[#481]](https://github.com/nf-core/smrnaseq/pull/481) - Fix [MIRTOP_STATS IndexError](https://github.com/nf-core/smrnaseq/issues/477) - Fix mirtop process execution when mirgenedb is used. +- [[#482]](https://github.com/nf-core/smrnaseq/issues/482) - Update documentation regarding MirgeneDB input files. ## v2.4.0 - 2024-10-14 - Navy Iron Boxer From d04cc55b2b1e8b67edcf801d23908aaf58b9392a Mon Sep 17 00:00:00 2001 From: atrigila <18577080+atrigila@users.noreply.github.com> Date: Tue, 12 Nov 2024 16:38:52 +0000 Subject: [PATCH 3/4] fix changelog --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index e2ee8bc3..f25e7a40 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -6,7 +6,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ## Dev vTBD - TBD - TBD - [[#481]](https://github.com/nf-core/smrnaseq/pull/481) - Fix [MIRTOP_STATS IndexError](https://github.com/nf-core/smrnaseq/issues/477) - Fix mirtop process execution when mirgenedb is used. -- [[#482]](https://github.com/nf-core/smrnaseq/issues/482) - Update documentation regarding MirgeneDB input files. +- [[#482]](https://github.com/nf-core/smrnaseq/pull/482) - Update documentation regarding MirgeneDB input files. ## v2.4.0 - 2024-10-14 - Navy Iron Boxer From 181039afdf80abb1ebc808908e2cf07935b85992 Mon Sep 17 00:00:00 2001 From: Anabella Trigila <18577080+atrigila@users.noreply.github.com> Date: Tue, 12 Nov 2024 14:43:08 -0300 Subject: [PATCH 4/4] Update docs/usage.md MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Nicolás Schcolnicov <90359308+nschcolnicov@users.noreply.github.com> --- docs/usage.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/usage.md b/docs/usage.md index 3549542a..c525e7b4 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -47,7 +47,7 @@ If MirGeneDB should be used instead it needs to be specified using `--mirgenedb` See examples in: [the test-datasets repository of nf-core](https://github.com/nf-core/test-datasets/tree/smrnaseq/MirGeneDB). -> [!NOTE] > `mirtop` is hard-coded to use the `pre` sequences rather than `pri`. Users must provide `pre` files from the start to ensure consistency between the FASTA and GFF files, as the coordinates in the GFF file are referenced to `pre` sequences. This also ensures that names in the BAM file will match those in the GFF. +> [!NOTE] > `mirtop` is hard-coded to use the `pre` sequences, which originate from the hairpin FASTA, rather than the `pri` sequences, which come from the mature FASTA. Users must provide `pre` files from the start to ensure consistency between the FASTA and GFF files, as the coordinates in the GFF file are referenced to `pre` sequences. This also ensures that names in the BAM file will match those in the GFF. ### Genome